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Laboratory Division
Institute of Pathology

The Institute of Pathology employs 42 members including a senior pathologist, residents, laboratory technicians, prosector, and administrative staff. The institute provides surgical and autopsy services, fosters an academic environment for training and education, and conducts basic and clinically oriented research.

 

Laboratory Services
The laboratory in the Institute of Pathology employs 15 technicians. The activities of the laboratory include routine Histology and Histochemistry, Cytology, Immunohistochemistry, PCR, in situ Hybridizatioin, FISH, and Electron Microscopy.

 

The Laboratory of Histology and Histochemistry prepares the routine histological sections of surgical material and autopsies. The laboratory also provides frozen section for rush diagnosis and prepares, on special request, histochemical staining. Histochemical staining is routinely performed for tissues such as Kidney, Liver, Lung biopsies, and Bone Marrow.

 

The Laboratory of Cytology tests the following specimen:

  • Cell collected from body fluid and mucoid material.
  • Cell obtained by scraping or brushing of epithelial cells and gynecological.
  • Samples obtained through aspiration of subcutaneous and deep tissue using fine needle techniques.

Each site has special collecting and technical requirements. In addition Immunocytochemistry and Histochemistry are performed to further extend the accuracy of the cytological investigation.

 

The Laboratory of Immunopathology performs Immunofluorescence Immunohistochemistry and in situ Hybridization. For immunofluorescent examination, the tissue is received fresh and preparations are performed on frozen section tissue. Immunohistochemical stains are performed on paraffin embedded and frozen tissue with large variety of antibodies. The immunohistochemical stains are performed upon request of the surgical pathologist. In situ Hybridization is performed mainly for identification of infectious agents such as Herpes Virus and Human PapillomaV irus of different types. PCR performed on formalin fixed paraffin embedded tissue. B & T cell rearrangement is evaluated in lymphoproliferative disorders.

 

At the Transmission Electron Microscopy Laboratory, Electron Microscopy in conjunction with Immunocytochemistry is an important technique for solving diagnostic problems in tumor pathology. In addition ultrastructure is carried on muscle biopsy, kidney biopsy, brush biopsy (ciliary dyskinesis), Lysosomal Disease, Viral Disease and others. The specimens are received after immediately being fixed in Glutaraldehyde.

 

We are currently involved in introducing modern technologies such as Fluorescent in Situ Hybridization (FISH), Comparative Genomic Hybridization (CGH) and in Situ PCR.

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